Date of Award
Master of Science (MS)
School of Biological Sciences
Tom M. Hammond
Meiotic silencing by unpaired DNA (MSUD) is a process observed in the model organism Neurospora crassa. During this process unpaired DNA between homologous chromosomes is detected and silenced, resulting in the suppression of unpaired genes. The effects of MSUD can be seen using phenotypic markers such as the Roundspore gene and evidence supports the existence of a physical search for unpaired DNA. However, the mechanism for detecting unpaired DNA remains uncertain. Previously, we have shown evidence that a Rad54-like protein, SAD-6 is required for the efficient completion of MSUD and may be necessary for the detection of unpaired DNA. Currently, we are working to determine the mechanism and proteins (along with SAD-6) that are involved in the homology search that detects unpaired DNA. SAD-6, and its homolog Rad54, share a conserved helicase domain and other similarities between their amino acid sequences suggesting they play a similar role within the nucleus. Rad54 is an essential protein throughout multiple stages of homologous recombination-based DNA repair. While it is uncertain whether homologous recombination is required for MSUD, we are investigating the possibility that SAD-6 shares similar biochemical activities with Rad54.
Smith, Zachary J., "Investigating the Roles and Interactions of Sad-6 within the Parameters of Meiotic Silencing by Unpaired Dna ( Msud )." (2016). Theses and Dissertations. 547.