Characterization of GTP and Aminoacyl-tRNA Binding to Eukaryotic Initiation Factor 2 and Elongation Factor 1

Document Type

Senior Thesis

Publication Date



Eukaryotic protein synthesis requires two protein factors which bind aminoacyl-tRNA in a GTP-dependent manner. One, initiation factor 2 (eIF-2), binds only the initiator Met-tRNAi and carries it to the 40S subunit of the ribosome. The other, elongation factor 1α (EF-1α) binds all other aminoacyl-tRNAs to the elongating 80S ribosome. The goal of this thesis is to characterize the structural aspects of these proteins.