Purification and Crystallization of the Yeast Elongation Factor eEF2
Document Type
Article
Publication Title
Acta crystallographica. Section D, Biological Crystallography
Publication Date
4-2002
Abstract
Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 A and belong to the space group P2(1)2(1)2(1). A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.
Recommended Citation
Jorgensen, Rene; Carr-Schmid, Anne; Ortiz, Pedro A.; Kinzy, Terri Goss; and Andersen, Gregers Rom, "Purification and Crystallization of the Yeast Elongation Factor eEF2" (2002). Faculty Publications – Biological Sciences. 38.
https://ir.library.illinoisstate.edu/fpbiosci/38
DOI
10.1107/s0907444902003001