Brain cancers, such as glioblastoma multiforme (GBM), exploit the body’s immune system to support tumor growth within their microenvironment. GBM utilizes various inflammatory pathways, including the CCR5 signaling pathway, to mediate the body’s response. In our study, we used the C6 glioma cell line, a widely recognized model for GBM, to examine the effect of the CCR5 antagonist maraviroc (MVR) on cell viability and intracellular nitric oxide (NO) levels. Using fluorescence microscopy, we visualized whether MVR induces changes in intracellular NO levels. This image is an overlay of brightfield and fluorescent images of C6 cells treated with MVR, with red fluorescence indicating the presence of NO in the cells. Fluorescence intensity was quantified using fluorescence spectroscopy, which revealed that MVR had no significant impact on NO levels. Our findings contribute to the ongoing study of MVR as a potential therapeutic agent for brain cancers and other neurological conditions.