Graduation Term

10-27-2022

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Department of Chemistry

Committee Chair

Jeremy JD Driskell

Committee Member

Jun-Hyun JHK Kim

Abstract

Many diagnostic tools for the accurate detection of diseases are time consuming and require multiple complex steps. Thus, there is a critical need to develop an alternative point of care test that provides rapid, reliable, and affordable results. Recently, surface-enhance Raman spectroscopy (SERS) has been developed as an analytical technique that enables low levels of detection and quantitative chemical analysis. Here, we exploit SERS in the development of a rapid diagnostic test, using human IgG as a model diagnostic biomarker. The vertical flow immunoassay system designed, utilizes filter paper embedded with gold nanoparticles (AuNP) to form a plasmonic paper. Capture antibody (anti-human IgG) is then immobilized onto the prepared plasmonic paper and inserted into a vertical flow device (syringe filter). Sample is passed through the filter paper and the target antigen (human IgG) is selectively captured by the immobilized antibody to form an antibody-antigen complex. Next, a functionalized AuNP (extrinsic Raman label) passes through the filter paper to label the captured biomarker molecules forming a sandwiched geometry. This sandwiched system enhances plasmonic coupling and SERS signal to provide highly sensitive detection of biomolecules. Importantly, this vertical flow approach can be performed in less than 5 minutes, meeting the demands for rapid point of care diagnostics.

DOI

https://doi.org/10.30707/ETD2022.20230207065834220248.999995

Page Count

88

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Available for download on Monday, February 03, 2025

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