Date of Award
Master of Science (MS)
Department of Chemistry
Jeremy JD Driskell
Jun-Hyun JHK Kim
Many diagnostic tools for the accurate detection of diseases are time consuming and require multiple complex steps. Thus, there is a critical need to develop an alternative point of care test that provides rapid, reliable, and affordable results. Recently, surface-enhance Raman spectroscopy (SERS) has been developed as an analytical technique that enables low levels of detection and quantitative chemical analysis. Here, we exploit SERS in the development of a rapid diagnostic test, using human IgG as a model diagnostic biomarker. The vertical flow immunoassay system designed, utilizes filter paper embedded with gold nanoparticles (AuNP) to form a plasmonic paper. Capture antibody (anti-human IgG) is then immobilized onto the prepared plasmonic paper and inserted into a vertical flow device (syringe filter). Sample is passed through the filter paper and the target antigen (human IgG) is selectively captured by the immobilized antibody to form an antibody-antigen complex. Next, a functionalized AuNP (extrinsic Raman label) passes through the filter paper to label the captured biomarker molecules forming a sandwiched geometry. This sandwiched system enhances plasmonic coupling and SERS signal to provide highly sensitive detection of biomolecules. Importantly, this vertical flow approach can be performed in less than 5 minutes, meeting the demands for rapid point of care diagnostics.
Ebbah, Eunice Ama, "Sers-Based Rapid Vertical Flow Immunoassay for Enhanced Point of Care Diagnostics" (2022). Theses and Dissertations. 1634.
Available for download on Monday, February 03, 2025