Date of Award

5-31-2023

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Department of Chemistry

First Advisor

Richard Nagorski

Abstract

The Nagorski group’s interest in carbinolamides stems from their role as an intermediate in the mechanism of the peptidylglycine-α-amidating monooxygenase (PAM) enzyme. Various carbinolamide roles have been studied as they pertain to biological processes, but no efforts have been put forth to understand how they react mechanistically. This group has set its focus on the kinetics of the breakdown of various carbinolamide derivatives in aqueous environments. The results gathered have been used to determine the mechanisms by which they break down in different regions of a full pH profile so that light may be shed on how to manipulate them in the enzymatic pathway. This study focuses on the synthesis and kinetic study of both ethyl-2-(benzoylamino)-2-hydroxyacetate and N-[1-hydroxy-2-oxo-2-(ethylamine)ethyl]benzamide derivatives. These neutral compounds were created in order to compare them to the previously studied α-hydroxyhippuric acid charged compound which was discovered to be a substrate for PAM. The mechanisms present in the hydroxide region for the derivatives synthesized in this study will be discussed and then compared to that of α-hydroxyhippuric acid.

Comments

Imported from Kerkemeyer_ilstu_0092N_12427.pdf

DOI

https://doi.org/10.30707/ETD2023.20231004061829117051.999963

Page Count

180

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Available for download on Monday, September 22, 2025

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